TopHat finds known and novelĮxon-exon splice junctions and is extremely fast due to its use of the Bowtie2 aligner. Bowtie and Bowtie2 were developed by Ben LangmeadĪ spliced alignment system for RNA-seq experiments. Genome with a Burrows-Wheeler index to keep its memory footprint small:Ģ.3 GB for the human genome. Sequences to the human genome at a rate of about 25 million reads per With high throughput (~1.5 to 2 million reads per second with the human reference genome in a 4-GPU computer), it is well suited to large-scale NGS data processing.Īn ultrafast, memory-efficient short read aligner that aligns short DNA Next-generation sequence alignment softwareĪrioc is a GPU-accelerated DNA short-read aligner for WGS and WGBS reads. If you're looking for the CHESS human gene database, it is at /chess Genome Assembly and Large-Scale Genome alignment.